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Project Description

Rationale

The project aims at the establishment of sustainable modern genomic and molecular techniques in the national marine partner institutes in order to support future ecosystem research initiatives. In order to reach this goal the microbial diversity research will be connected with state of the art transcriptome and proteome analysis techniques as well as metagenomic in situ approaches. The integration of these different techniques will enable us to methodically determine the complexity of microbial dominated metabolic circles in marine ecosystems in a new dimension.

 

The project focuses on the development of new molecular biological techniques for the determination of complex metatranscriptomes and metaproteomes of marine microbial assemblages. The suitability of these techniques as future tools for the analyses of bacterial mediated ecosystem functions will be validated at selected marine model locations. The comprehensive analysis of the genomic diversity and activity of marine microorganisms is a key for a better understanding of climate changes and other anthropogenic influences (e.g. eutrophication) on biogeochemical nutrient cycles.

 

 The comprehensive, cultivation-independent determination of the composition and function of marine microbial communities by means of a combined metagenomic, metatranscriptomic and metaproteomic approach will facilitate new knowledge on ecophysiological activities and adaptations of microorganisms in marine habitats. On the basis of detailed functional genome analyses of selected marine model bacteria environmentally relevant marker genes will be determined. By the parallel acquisition of biogeochemical and genetic parameters including the data integration an improved monitoring of marine ecosystems is expected (Fig. 1).

 

 

MIMAS Organigram
Fig. 1